Lung cancer accounts for 80% of all cancers and is the leading cause of cancer death in China. Most cases of lung cancer are non-small cell lung cancer, which is the most common type of lung cancer. To overcome the limitations of surgical treatment and chemotherapy for lung cancer, we constructed a lentivirus that could specifically target miR-675-5p. The influence of miR-675-5p on lung cancer cell proliferation was evaluated using MTT and colony formation assays. The migration and invasion of lung cancer cells were evaluated using transwell assays. In addition, the expression of target proteins and downstream molecules was evaluated by western blotting and immunohistochemical staining.
The expression of miR-675-5p was analyzed by real-time quantitative PCR (qRT-PCR). The effect of miR-675-5p on proliferation was evaluated through MTT and colony formation assays, and cell migration and invasion were evaluated through transwell assays. The expression of target proteins and downstream molecules was analyzed by western blotting and immunohistochemical staining. The luciferase reporter assay was used to assess the target genes of miR-675-5p in non-small cell lung cancer cells.
Materials and methods: MiR-675-5p was transfected into A549 and H1299 cells to validate the effect of miR-675-5p on NSCLC cell growth in vitro. MiR-675-5p was introduced into nude mice to assess tumorigenic and metastatic ability. The target genes of miR-675-5p were investigated through bioinformatic analysis and luciferase reporter assays. The expression of SRC and paxillin was evaluated using western blotting and immunohistochemical staining, respectively. The expression of EMT-associated proteins was analyzed through western blotting.
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